distribution of il-28b genotypes in patients with hepatitis c and healthy individuals in jahrom city

aim : the purpose of this study was to compare the distribution of interleukin (il)-28b genotypes between iranian healthy individuals and patients with chronic hepatitis c based on the genotype. background : polymorphisms in the region of il-28b gene have been identified as the strongest genetic pretreatment predictor of sustained virological response (svr) in hepatitis c infection. patients and methods : in this study, 147 patients with chronic hepatitis c and 80 healthy individuals were included. the il-28b rs12979860 and rs8099917 polymorphisms were genotyped by pcr-rflp method and the frequency of il-28b polymorphisms with respect to hcv genotypes was also determined. results : the frequencies of rs12979860 tt, cc and ct genotypes in the chronic hepatitis c patients and healthy individuals were as follows: 10.8% vs. 11.3%, 38.7% vs. 46.2% and 50.3% vs. 42.5%. also, the frequencies of rs8099917 tt, gg and gt genotypes in the chronic hepatitis c patients was 61.9%, 6.1% and 32% and in controls was 47.5%, 11.2% and 41.3%. the differences in the distribution of rs12979860 genotypes and alleles between hcv genotype 1 and hcv genotype 3a infected patients were statistically significant. conclusion : the rs12979860 c allele is the favorable allele for the spontaneous clearance of hcv. it seems that the impact of il-28b polymorphism on the spontaneous clearance of hcv genotype 3 is more prominent than hcv genotype 1, which results in the observation of higher rs12979860 c allele frequency in chronic hepatitis c patients with hcv genotype 3 than hcv genotype 1.